This article discusses the use of Python language pySerial library on Raspberry Pi or RPi both 3rd and 4th generation to connect to the serial port. It can be done in 2 ways, the first is to use hardware like ET-CONV10/RS232 HAT that has been written in the book and with the use of a USB port connected to a converter to be a serial communication port (USB to Serial Port) as shown in Figure 1. This article uses the second method to run pySerial to check how many serial ports are connected and what are their names as an example in Figure 8.